《植物生理学报》 2010, 46(5): 427-430

外源钙对高温强光胁迫下小麦叶中蛋白激酶活性和D1 蛋白磷酸化的影响

李利红^1,2, 梁书荣³, 曲小菲³, 赵会杰^3,*

1 河南农业大学农学院, 郑州450002; 2 郑州牧业工程高等专科学校药物工程系, 郑州450011; 3 河南农业大学生命科学学院,

通信作者：赵会杰；E-mail: zhaohj303@163.com；Tel: 0371-63555319

摘　要：

检测灌浆期叶面喷施 10 mmol•L-1CaCl 和1 mmol•L-1 蛋白激酶抑制剂(FSBA)对高温强光胁迫下小麦叶中蛋白激酶活 性和D1蛋白磷酸化影响的结果表明, CaCl2可提高而蛋白激酶抑制剂则抑制高温强光下蛋白激酶活性和D1蛋白含量及其 磷酸化水平。

关键词：小麦; 高温强光; Ca2+; 蛋白激酶; D1 蛋白磷酸化

收稿：2009-12-09   修定：2010-01-29

资助：国家自然科学基金项目(30971725, 30671214)。

Effects of Ca²⁺ on the Activiy of Protein Kinases and Phosphorylation of D1 Protein in Wheat (Triticum asetivum L.) Leaves under Heat and High Irradiance Stress

LI Li-Hong^1,2, LIANG Shu-Rong³, QU Xiao-Fei³, ZHAO Hui-Jie^3,*

¹College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China; ²Department of Pharmaceutical Engineering, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China; ³College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China

Corresponding author: ZHAO Hui-Jie; E-mail: zhaohj303@163.com; Tel: 0371-63555319

Abstract:

This article aimed at proving the effects of 10 mmol·L^-1 CaCl₂ and 1 mmol·L^-1 FSBA on the protein kinases activity and D1 protein phosphorylation in leaves of wheat (Triticum asetivum) at filling stage under high temperature and irradiance stress. Results showed that pretreatment with CaCl₂ improved remarkably the activity of protein kinases and increased the content of D1 protein and it’s phosphorylation, while FSBA had the reverse effects.

Key words: wheat; high temperature and irradiance stress; Ca²⁺; protein kinase; phosphorylation of D1 protein